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WebFeb 11, 2024 · Here, we identify that Drosophila Pif1A acts as a key regulator for sperm individualization. Loss of Pif1A leads to male sterility associated with irregular individualization complex and empty... WebMay 20, 2016 · The expression of several actin-related proteins in mammalian sperm (some of which are testis-specific) suggests that actin polymerization and depolymerization might be involved in certain aspects of sperm function. A summary of the actin-binding proteins reported in mammalian sperm and the possible function is shown in Table 7.1. WebApr 12, 2024 · The fluorescent agent FITC-phalloidin, which stains F-actin filaments, was used to measure actin polymerization in sperm cells during capacitation. Sperm samples were collected by centrifugation (360 × g for 5 min), washed twice in PBS and fixed with 1% PFA in PBS (Santa Cruz Biotechnology Inc., Dallas, TX, USA) for 10 min. After fixation, the ... pokemon fighters ex uncopylocked