Biotinylated mouse tim4-fc

Author: wbzm

August undefined, 2024

WebCD45 in Mouse Thymus. CD45 was detected in perfusion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD45 Biotinylated Monoclonal Antibody (Catalog # … WebThe cells were then stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b (Cat. No. 550993/561114) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse TIM-4 antibody (Right Panel). Two-color flow cytometric contour plots showing the correlated expression patterns of TIM-4 (or Ig Isotype control staining ...

Tim-4 (mouse):Fc (human) (recombinant protein) - Order from …

WebOutline: This product is for research use only. Do not administer it to human. This is a recombinant fusion protein; the extracellular domain of mouse TIM4 (TIMD4) is linked to the N-terminus of human IgG 1 Fc domain … WebSep 23, 2016 · Nevertheless, Tim4-Fc-conjugated beads were still able to isolate sEVs efficiently from 10K sup of pMac which abundantly express … high quality carpet tile

APC anti-mouse Tim-4 antibody, Tim-4, RMT4-54 - BioLegend

WebMar 15, 2024 · Then concentrated CM were incubated with Streptavidin Magnetic Beads (60 mg) accompanied by additional 1 μg of biotinylated mouse Tim4-Fc, 350 μL Exosome Capture Immobilizing Buffer, and 50 μL Exosome Binding Enhancer overnight at 4 °C. The next day, the beads were washed with washing buffer for three times and the bound EVs … WebMouse Tim4–Fc was prepared as described . To produce mProS and mGas6, full-length cDNAs for mProS (Gene ID: 19128) and mGas6 (Gene ID: 14456) were prepared by RT-PCR with mRNA from mouse liver and … WebSpecificity / Sensitivity. Anti-mouse IgG (H+L), (Biotinylated) reacts with the heavy and light chains of most mouse immunoglobulins. No cross-reactivity to other serum proteins … how many bytes is 1 mb

Mouse macrophages show different requirements for …

WebThis antibody was developed for use in flow cytometry. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with … WebMay 19, 2024 · 2.3 TIM4-Affinity Beads. 1. Mouse TIM4-Fc protein. 2. Maleimide-PEG11-Biotin: 25 mM Maleimide-PEG11-Biotin in dimethylsulfoxide (DMSO). To prepare 250 … high quality carpetingWebadjective. bio· tin· y· lat· ed ˌbī-ə-ˈti-nə-ˌlā-təd. : combined with biotin. The PCR reaction uses biotinylated primers to define a sequence of approximately 207 nucleotides within … high quality cartridge

"WebMeaning of biotinylated. What does biotinylated mean? Information and translations of biotinylated in the most comprehensive dictionary definitions resource on the web. " - Biotinylated mouse tim4-fc

Biotinylated mouse tim4-fc

Tim4, a macrophage receptor for apoptotic cells, binds …

WebBackground: IgG1. R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. WebMouse Ig cocktail. Formulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation. The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C.

Did you know?

WebTim-4 can isolate extracellular vesicles from different species (human, mouse, rat, etc.) containing phosphatidylserine (PS) at their surface. Biological Activity Measured by its … WebRecombinant Mouse TIM-4 (Catalog # 2826-TI) inhibits anti-CD3-induced proliferation of stimulated human T cells. Human T cells cultured for 72 hours with PHA are incubated …

WebFeb 9, 2024 · Needle-like multi-walled carbon nanotubes (MWCNTs) are engulfed by macrophages, which cause inflammation and asbestosis-like pathogenesis in rodents; however, how macrophages recognize … WebAug 31, 2024 · The EVs were isolated from the sera of PC patients or NC in cohort-1 using the Magcapture Exosome Isolation Kit (Fujifilm) according to the manufacturer’s protocol. In brief, 0.6 mg of streptavidin magnetic beads, bound with 1 µg of biotinylated mouse Tim4-Fc, was added to the serum supplement and rotated overnight at 4 °C.

WebMouse Tim4–Fc was prepared as described . To produce mProS and mGas6, full-length cDNAs for mProS (Gene ID: 19128) and mGas6 (Gene ID: 14456) were prepared by RT … WebMouse TIM-4 Antibody Summary Species Reactivity Mouse Specificity Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with …

WebFUJIFILM biotinylated mouse tim4 fc Biotinylated Mouse Tim4 Fc, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed …

WebMar 12, 2014 · TIM4 is the main receptor mediating the phagocytosis of PtdSer-bearing targets in murine macrophages. Bone marrow-derived macrophages (BMDMs) from TIM4 − / − mice were reported to have a reduced ability to engulf apoptotic bodies (Martinez et al., 2011).This observation suggests that TIM4 may be the primary PtdSer receptor in these … high quality carrageenan powderWebMonoclonal Hamster‑Armenian anti‑Mouse TIMD4 / TIM4 / TIM‑4 Antibody (FITC, IF) LS‑C663029. ... FC: Cells were incubated on ice for 30 min with 1 ug/ml biotinylated Kat5-18 in 100 ul staining solution (PBS containing 2% FCS), followed by incubation with 2 ug/ml Allophycocyanin (APC)-Cyanine 7 (Cy7)-conjugated streptavidin and 2 ug/ml ... high quality cat food at petcoWebJun 29, 2024 · An ELISA plate (AGC Techno Glass, Shizuoka, Japan) was coated with 1 μg/mL recombinant mouse TIM4-Fc protein (FUJIFILM Wako Pure Chemical)/50 mM carbonate-bicarbonate buffer (pH 9.6) and ... high quality carry-on luggage high quality cat pelt p99Weblipids, including PtdCho, PtdEtn, and sphingomyelin. Tim4–Fc, a fusion protein of the extracellular region of mTim4 and hu-man IgG1–Fc (12), showed a similar specificity for PtdSer, with a three to eight times stronger affinity than that of mProS or mGas6 for PtdSer (Fig. 1). Interaction Between Mouse TAM Ligands and Their Receptors. We high quality casino signageWebTo produce an mAb against Tim4, Armenian hamsters were immunized with Tim4-Fc, in which the extracellular region of mouse Tim4 was joined to human IgG Fc region (11). Lymphocytes from the immunized hamsters were fused with a mouse myeloma NSObcl2 (17). One hybridoma (Mat4-9-5) that was suitable for immunohistochemical staining, how many bytes is 24 kilobytesWebNov 10, 2024 · Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified) FCM-M82W5: HEK293: Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His, Avitag™ (MALS & BLI verified) FCM-M82W6 ... high quality carpets brands